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Microencapsulation of broccoli sulforaphane using whey and pea protein: in vitro dynamic gastrointestinal digestion and intestinal absorption by Caco-2-HT29-MTX-E12 cells

Microencapsulation of broccoli sulforaphane using whey and pea protein: in vitro dynamic gastrointestinal digestion and intestinal absorption by Caco-2-HT29-MTX-E12 cells

ABSTRACT: Sulforaphane, an organosulfur phytochemical, has been demonstrated to have significant anticancer potential in both in vitro and in vivo studies, exhibiting mechanisms of action that include inducing apoptosis, inhibiting cell proliferation, and modulating...
Comprehensive protocol for preparing diatom cell samples and associated bacterial consortia for scanning electron microscopy

Comprehensive protocol for preparing diatom cell samples and associated bacterial consortia for scanning electron microscopy

ABSTRACT: Meticulous sample preparation and strict adherence to preservation procedures are essential for electron microscopy investigations, which enable accurate capture of organisms’ morphology, size, and potential interactions within the sample. Here, we present a...
Microbe-mineral interactions within kimberlitic fine residue deposits: impacts on mineral carbonation

Microbe-mineral interactions within kimberlitic fine residue deposits: impacts on mineral carbonation

ABSTRACT: The observation of photosynthetic biofilms growing on the Fine Residue Deposit (FRD) kimberlite produced by the Venetia Diamond Mine, Limpopo, South Africa suggests that processed kimberlite supports bacterial growth. The presence of this biofilm may aid in...
Bacteria conjugate ubiquitin-like proteins to interfere with phage assembly

Bacteria conjugate ubiquitin-like proteins to interfere with phage assembly

ABSTRACT: Multiple immune pathways in humans conjugate ubiquitin-like proteins to virus and host molecules as a means of antiviral defense. Here we studied an anti-phage defense system in bacteria, comprising a ubiquitin-like protein, ubiquitin-conjugating enzymes E1...
Scalable Substrate Development for Aqueous Biological Samples for Atom Probe Tomography

Scalable Substrate Development for Aqueous Biological Samples for Atom Probe Tomography

ABSTRACT: Reliable and consistent preparation of atom probe tomography (APT) specimens from aqueous and hydrated biological specimens remains a significant challenge. One particularly difficult process step is the use of a focused ion beam (FIB) instrument for...
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